Exploiting the Triple Response of Arabídopsís To ldentify Ethylene-Related Mutants

نویسنده

  • Plinio Guzman
چکیده

Alterations in the response of dark-grown seedlings to ethylene (the “triple response”) were used to isolate a collection of ethylene-related mutants in Arabidopsis thaliana. Mutants displaying a constitutive response (etol ) were found to produce at least 40 times more ethylene than the wild type. The morphological defects in etiolated etol-l seedlings reverted to wild type under conditions in which ethylene biosynthesis or ethylene action were inhibited. Mutants that failed to display the apical hook in the absence of ethylene (hls7) exhibited reduced ethylene production. In the presence of exogenous ethylene, hypocotyl and root of etiolated hlsl-7 seedlings were inhibited in elongation but no apical hook was observed. Mutants that were insensitive to ethylene (einl and ein2) produced increased amounts of ethylene, displayed hormone insensitivity in both hypocotyl and root responses, and showed an apical hook. Each of the “triple response” mutants has an effect on the shape of the seedling and on the production of the hormone. These mutants should prove to be useful tools for dissecting the mode of ethylene action in plants.

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تاریخ انتشار 2002